Lady Anne has a wide repertoire of courtly songs in English, French, Spanish, Italian or Latin. She accompanies herself either on the lute or the Spanish vihuela a mano. This instrument, shaped like a small guitar, is the Spanish equivalent of the lute, having similar double courses of strings and tuned as the lute. Both instruments also offer a great range of solo music. She plays arrangements of part songs, singing one part and simultaneously playing up to four more on her instrument. These arrangements are handwritten and it is from books such as hers that most early music has been preserved. When demonstrating the Elizabethan period it is possible to show some of the earliest printed music available: beautifully bound facsimile books of music for the vihuela by the Spanish composers Luis Milan (1535) and Luis de Narvaez (1538).

Occasionally she plays on a renaissance flute – a simple, keyless wooden instrument or recorder. The addition of a small consort of renaissance recorders, made by Phil Bleazy, used to give much more variety to the musical performances and made it possible to provide a strong accompaniment for dancing, the lute or vihuela, as chamber instruments, being insufficiently loud for the purpose.